LOCUS       Type_A_IS26-fosA3-IS26_unit4137 bp   DNA   linear       03-OCT-2020
DEFINITION  Citrobacter freundii.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Unclassified.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of Type A IS26-fosA3-IS26 unit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-OCT-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
     repeat_region   complement(1..14)
                     /gene="IRR_IS26"
     CDS             complement(53..757)
                     /gene="tnpA"
                     /codon_start=1
                     /product="IS26 transposase"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     repeat_region   complement(807..820)
                     /gene="IRL_IS26"
     CDS             1143..1559
                     /gene="fosA3"
                     /codon_start=1
                     /product="Fosfomycin resistance protein FosA3"
                     /translation="MLQGLNHLTLAVSDLASSLAFYQQLPGMRLHASWDSGAYLSCGA
                     LWLCLSLDEQRRKTPPQESDYTHYAFSVAEEEFAGVVALLAQAGAEVWKDNRSEGASY
                     YFLDPDGHKLELHVGNLAQRLAACRERPYKGMVFFD"
     CDS             complement(1564..2082)
                     /gene="orf1"
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MRLAPRPFFALCVLVFIAAAAFSAWRLLPADNAGVMSCSTKGIM
                     RFENMDKENVNGNIHFNFGAQGKGSMVVEGYTDSAAGWLYLQRYVKFTYSSKRISATE
                     RHYRINQWESSASSIDESPNVIFDYFMREMSDSHDGLFLNAQKLNEKAILLSSINSPL
                     WICTLKSGSKLN"
     CDS             complement(2082..2870)
                     /gene="orf2"
                     /codon_start=1
                     /product="Transcriptional regulator"
                     /translation="MRYNINARFIYDATDGTLTLPQSDEPDSQLSLTASALFNYFLRH
                     TEIVSREDVLKKVWDDNGLTSSNSNLNQYLSMLRKTFRHYGVDNIIVTVSRGYLQLNP
                     EVIIEALDDAPPVSPQQTPAEPPPVTTTPDAPRSAHARGMCWYMAGACLLTIALLLVA
                     FSLVGISESQPIALTRLSHSQCELLASDEMRRSVSEESYGKNFDEVRQRLNIACKPGE
                     RFLFFYGDRLETNGLGRVFLAHCAMHEDNPFSYCDNYFYYSWKP"
     CDS             complement(2890..3330)
                     /gene="orf3"
                     /codon_start=1
                     /product="TetR family transcriptional regulator"
                     /translation="MQQCRKGGLIHHFPNKQALIFALFARLLAIMEEAITALMQQDGV
                     SYGRFTRAYLNYLADLTDTHESRQLMVLSLAMPDEPVLRKCWRDWMLEKLAQGDELDN
                     SPTGTLVRYAADGIWLSELTEGITMSADHRRALVDSLNKMTLPA"
     repeat_region   complement(3318..3331)
                     /gene="IRR_IS26"
     CDS             complement(3370..4074)
                     /gene="tnpA"
                     /codon_start=1
                     /product="IS26 transposase"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     repeat_region   complement(4124..4137)
                     /gene="IRL_IS26"
ORIGIN      
        1 ggcactgttg caaagttagc gatgaggcag ccttttgtct tattcaaagg ccttacattt
       61 caaaaactct gcttaccagg cgcatttcgc ccaggggatc accataataa aatgctgagg
      121 cctggccttt gcgtagtgca cgcatcacct caataccttt gatggtggcg taagccgtct
      181 tcatggattt aaatcccagc gtggcgccga ttatccgttt cagtttgcca tgatcgcatt
      241 caatcacgtt gttccggtac ttaatctgtc ggtgttcaac gtcagacggg caccggcctt
      301 cgcgtttgag cagagcaagc gcgcgaccat aggcgggcgc tttatccgtg ttgatgaatc
      361 gcgggatctg ccacttcttc acgttgttga ggattttacc cagaaaccgg tatgcagctt
      421 tgctgttacg acgggaggag agataaaaat cgacagtgcg gccccggctg tcgacggccc
      481 ggtacagata cgcccagcgg ccattgacct tcacgtaggt ttcatccatg tgccacgggc
      541 aaagatcgga agggttacgc cagtaccagc gcagccgttt ttccatttca ggcgcataac
      601 gctgaaccca gcggtaaatc gtggagtgat cgacattcac tccgcgttca gccagcatct
      661 cctgcagctc acggtaactg atgccgtatt tgcagtacca gcgtacggcc cacagaatga
      721 tgtcacgctg aaaatgccgg cctttgaatg ggttcatgtg cagctccatc agcaaaaggg
      781 gatgataagt ttatcaccac cgactatttg caacagtgcc gtaattattt aggatatagc
      841 tctgtgattt agtcttttta gggcgtttat ttatgtcttt atgctgtaat gtcggtgctg
      901 atttggtctt caataaggcg atttttagcc cgctttttcc ggggggatga aatgttattg
      961 gattgctaaa tgatttcaaa aaaggggtaa catagagcgg ggattagtgt ggcgaggcgc
     1021 aggtttccga tggcaggcta aacgcaaaaa atgcgctttt tagccggtga tgaggtgagg
     1081 ccgggagggg atgcgtcgcc gatcacagtt tacaacaggg tttgataacg ggaggaaaag
     1141 tcatgctgca gggattgaat catctgacgc tggcggtcag cgatctggcg tcaagcctgg
     1201 cattttatca gcagttacct ggaatgcgcc tgcacgccag ctgggatagc ggagcctatc
     1261 tctcctgtgg ggcgctgtgg ctgtgcttgt cgctggatga gcagcggcgt aaaacgcccc
     1321 ctcaggaaag cgactatacc cactacgcct tcagcgtggc ggaagaagag tttgccgggg
     1381 tggtggctct gctggcgcag gcgggggctg aggtatggaa agataaccgc agtgaagggg
     1441 cgtcttacta ttttctcgac cctgacggcc ataagctgga gctgcatgtg gggaatctgg
     1501 cgcagcggct ggccgcctgt cgcgaacgcc cctacaaggg gatggtcttt tttgattgac
     1561 gggttagttc agcttactgc cggatttcaa cgtgcagatc cacagcggtg aattgataga
     1621 gctgagcaag atagcctttt cgttgagctt ctgagcgttg agaaacagcc cgtcgtggct
     1681 gtcggacatc tcgcgcataa agtagtcgaa aatcacgttc ggcgattcat caatcgagga
     1741 ggcgctcgat tcccactgat tgatgcggta atgacgctcg gtggccgaga tgcgtttgct
     1801 gctgtaggta aacttcacgt agcgttgcag gtagagccag ccggcggcgg agtcggtgta
     1861 accttcgacc accatcgagc ctttaccctg cgcgccaaaa ttgaagtgaa tattgccgtt
     1921 aacgttctct ttgtccatgt tctcaaagcg catgatgccc ttagtggaac agctcatgac
     1981 acccgcgttg tcggcaggca gcagccgcca ggcgctaaac gcggcggcgg caataaatac
     2041 cagaacgcag agggcaaaga acggacgcgg cgcgagtctc attacggctt ccaggagtag
     2101 taaaagtagt tatcgcaata gctgaacggg ttatcttcat gcatggcgca gtgggcaagg
     2161 aacacgcgcc ccagcccgtt ggtttcgagc cgatcgccgt agaagaaaag aaaacgttca
     2221 ccgggtttac aggcaatatt cagccgctgg cgtacctcat cgaagttttt gccgtaggac
     2281 tcttcactga ccgaacgtcg catttcatcg ctggccagca gttcgcactg gctatggctg
     2341 aggcgggtca gcgcgatagg ctgggattca ctgatgccga ccagactgaa ggcaaccaga
     2401 agcagggcaa tggtgagcaa gcaggctccg gccatgtacc agcacatccc gcgagcatgt
     2461 gcggagcgcg gtgcgtctgg cgttgtcgta acgggcggcg gttcggcagg cgtctgttgt
     2521 ggtgaaaccg gcggcgcgtc gtccagcgct tcgataatga cctcgggatt aagctgcaaa
     2581 tagccgcgcg agacggtgac gatgatgtta tcgacgccgt aatggcgaaa ggtcttccgc
     2641 agcatgctga gatactgatt cagattgctg ttggatgagg ttaatccatt gtcatcccag
     2701 acttttttca gaacatcttc ccggctgaca atctcggtat ggcgcagaaa atagttaaac
     2761 agggcgctgg cggtgaggga tagctggctg tccggttcat cgctttgcgg cagcgtcagg
     2821 gttccgtcgg tggcgtcgta gataaaacgg gcattgatgt tgtagcgcat cgcggcctca
     2881 gtctgtgctt cacgcgggaa gcgtcatttt attcagggag tcaaccagcg cgcgacggtg
     2941 gtcggcgctc atggtgatcc cttccgtcaa ctccgaaagc cagatgccgt cagcggcgta
     3001 gcgcaccagc gtgccggttg ggctgttatc cagctcatcg ccctgcgcca gtttctccag
     3061 catccagtcg cgccagcatt tgcgcagcac cggttcatcg ggcattgcca gcgacaaaac
     3121 cataagctgg cggctttcat gggtgtccgt gagatccgcc agatagttca gataggcgcg
     3181 ggtaaaccgg ccatagctca cgccatcctg ttgcatcagc gcggtaatcg cttcttccat
     3241 gattgccagc agacgcgcga acagggcgaa tattagcgcc tgcttgttcg ggaaatggtg
     3301 gataaggccg cctttacggc actgttgcaa agttagcgat gaggcagcct tttgtcttat
     3361 tcaaaggcct tacatttcaa aaactctgct taccaggcgc atttcgccca ggggatcacc
     3421 ataataaaat gctgaggcct ggcctttgcg tagtgcacgc atcacctcaa tacctttgat
     3481 ggtggcgtaa gccgtcttca tggatttaaa tcccagcgtg gcgccgatta tccgtttcag
     3541 tttgccatga tcgcattcaa tcacgttgtt ccggtactta atctgtcggt gttcaacgtc
     3601 agacgggcac cggccttcgc gtttgagcag agcaagcgcg cgaccatagg cgggcgcttt
     3661 atccgtgttg atgaatcgcg ggatctgcca cttcttcacg ttgttgagga ttttacccag
     3721 aaaccggtat gcagctttgc tgttacgacg ggaggagaga taaaaatcga cagtgcggcc
     3781 ccggctgtcg acggcccggt acagatacgc ccagcggcca ttgaccttca cgtaggtttc
     3841 atccatgtgc cacgggcaaa gatcggaagg gttacgccag taccagcgca gccgtttttc
     3901 catttcaggc gcataacgct gaacccagcg gtaaatcgtg gagtgatcga cattcactcc
     3961 gcgttcagcc agcatctcct gcagctcacg gtaactgatg ccgtatttgc agtaccagcg
     4021 tacggcccac agaatgatgt cacgctgaaa atgccggcct ttgaatgggt tcatgtgcag
     4081 ctccatcagc aaaaggggat gataagttta tcaccaccga ctatttgcaa cagtgcc
//
